![]() ![]() ![]() Setting up large layouts: Really useful tools for the display of many graphs are placeholders and Layout Grids.Īnnotating data: Legends and adding graph annotation allows you to add additional information about your samples including statistics and keywords. Visualization options: Here are a handful of visualization options that can be accessed by right-clicking on a graph including Backgating, which reveals the effect of every gate in the gating tree of a multi-generation gated population, and adjunct histograms which displays univariate (histogram) displays of data in a bivariate graph.Ĭomparing samples: A great way to compare many samples is through the use of Overlays and Multigraph Overlay. Since GITR stimulation is known to affect both Teff and Tregs, this assay can be used to test two variables: (1) the effect of GITR stimulation on modulating the suppressive function of Tregs and (2) the effect of GITR stimulation on modulating the activation state of effector T cells. The Layout Editor has a number of options to display your graphs (charts) and annotate your data.Ĭhanging visualization and annotation: The central place to go to edit charts and add annotation is the Graph Definition Window, which can be accessed by double-clicking on a graph. FlowJo for Antibody Titrations: SI and Concatenation UWCCC Flow Lab 05/05/16 1.0 Make a fourth column to calculate the Separation Index. In this protocol, we used GITRL as the immunomodulatory agent. ![]()
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